Journal: Cell Genomics

Article Title: exRNA-eCLIP intersection analysis reveals a map of extracellular RNA binding proteins and associated RNAs across major human biofluids and carriers

doi: 10.1016/j.xgen.2023.100303

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Article Snippet: Human Bone Marrow Stromal Cells Derived in ACF Medium (female) , STEMCELL Technology , Cat#70071.

Techniques: Recombinant, Cell Attachment Assay, Clinical Proteomics, Staining, Blocking Assay, Western Blot, Bicinchoninic Acid Protein Assay, Derivative Assay, Software, Expressing

Journal: Frontiers in Immunology

Article Title: Modeling human natural killer cell development and drug response in a microfluidic bone marrow model

doi: 10.3389/fimmu.2025.1499397

Figure Lengend Snippet: Setup of the NK cell bone marrow model in the HUMIMIC Chip2. (A) Exploded view of the HUMIMIC Chip2 showing the three structural layers: the polycarbonate adapter plate housing the culture compartments, the PDMS layer housing the microfluidic circulation, and the bottom glass layer closing the circulation and allowing microscopic observation. (B) 2D view of the HUMIMIC Chip2 microfluidics: the outer compartment houses the bone marrow model; the inner compartment is used as a medium compartment and as a sampling point for hematopoietic cells. (C) Progenitor populations until Stage 2B of NK cell development in CD34+ starting cell pool from three donors on day 0. (D) Exemplary microscopic images of a medium compartment on day 7 with 35% confluency and on day 21 with 100% confluency, scale is 1mm. (E) Time schedule of the NK differentiation assay in the Chip2 system with weekly sampling points for FC analysis and media exchanges every 2–3 days. (F) Used surface marker to distinguish NK cell maturation stages adapted from Abel et al. , Cichoki et al. and Bi et al. . NK development goes through a stepwise differentiation starting from hematopoietic stem cells to CLP, NK cell precursors (NKP), and immature NK cells (iNK) toward mature NK cells (mNK).

Article Snippet: Cryopreserved human bone marrow stromal cells (Stemcell Technologies, Vancouver, BC, Canada) were thawed and cultured in MSC medium consisting of DMEM + 10% FCS + 1% penicillin-streptomycin (Corning, Corning, NY, USA).

Techniques: Sampling, Differentiation Assay, Marker

Journal: Frontiers in Immunology

Article Title: Modeling human natural killer cell development and drug response in a microfluidic bone marrow model

doi: 10.3389/fimmu.2025.1499397

Figure Lengend Snippet: Characterization of lineage differentiation in the bone marrow model. (A) Sampled cell counts of circuits over time from the circulation and harvested cell counts form the ceramic scaffold at the end of the experiment. Mean values ± SD of individual cell populations are shown as stacked bar graphs from three CD34+ donors with two chips (N = 3, n = 2). (B) Gating strategy for analysis of dendritic cells in the hematopoietic cell pool sampled on day 35 of chip culture. (C) Percentage of NK cells, granulocytes, monocyte, inflammatory DCs, myeloid/conventional DC1 and myeloid/conventional DC2 sampled at the end of the assay on day 35. Mean values ± SD of individual cell populations from three CD34+ donors (N = 3, n = 1) are shown. (D) Proliferation rate of stage 1, 2, 3, 4, and 5 NK cells in circulation on day 28 and day 35 of the assay. Mean values ± s.e.m of individual cell populations from one CD34+ donors with six chips (N = 1, n = 6) are shown. (E) Sampled cell counts of the lymphoid/NK cell lineage from the circulation over time. Mean values ± SD of individual cell populations from three experiments with in total four CD34+ donors with two to three chips (N = 4, n = 2–3) are shown.

Article Snippet: Cryopreserved human bone marrow stromal cells (Stemcell Technologies, Vancouver, BC, Canada) were thawed and cultured in MSC medium consisting of DMEM + 10% FCS + 1% penicillin-streptomycin (Corning, Corning, NY, USA).

Techniques: